Scientists exploit organisms' needs in order to track 'vitamin mimics' in bacteria
January 21, 2016 
RICHLAND, Wash. - Microbes need their vitamins just like people do. Vitamins help keep both organisms healthy and energetic by enabling proteins to do their work. For bacteria, a dearth of vitamins can spell death.
Now scientists at the D.O.E.'s Pacific Northwest National Lab have made a "vitamin mimic" - a molecule that looks and acts just like the natural vitamin to bacteria, but can be tracked and measured by scientists in live cells. The research offers a new window into the inner workings of living microbes that are crucial to the world's energy future, wielding great influence in the planet's carbon and nutrient cycle and serving as actors in the creation of new fuels.
Vitamins are a powerful currency for researchers seeking to compel microbes to give up their secrets.
"We have a lot to learn about how microbes accumulate and use nutrients that are necessary for their survival and growth. This provides a window for doing so," said chemist Aaron Wright, the corresponding author of the study published in ACS Chemical Biology.
"Perhaps we will be able to make a microbial community do what we want, by controlling its access to a specific nutrient," Wright added.
To control the bacteria via vitamins, Wright and his team have to know what other proteins in the cell the vitamins are consorting with, and where and when.
Think of a planner analyzing emergency services for a large city. Knowing that an ambulance enters the city occasionally and transports some people somewhere, for instance, is not nearly as useful as knowing the precise address of the caller, the identity of the injured, and the location of the nearest hospital.
It's the same for scientists trying to understand microbial cells. While a cell is infinitesimally small, the activity within resembles the hustle and bustle of a large city, with many functions within carried out by thousands of entities. Knowing precisely which vitamins aid which proteins, under what circumstances, to keep things running is a must if scientists are to maximize microorganisms for energy production and other processes.
"Microbial communities are organized based on their ability to get the resources they need to survive and grow," said Wright. "We need to understand how the availability of nutrients, like vitamins, helps determine the structure of a microbial community as a step toward controlling that community in ways we would like to be able to do."
An anchor for pond scum
Wright's team studied the bacterium Chloroflexus aurantiacus J-10-fl, which is a common member of microbial mats - gloopy natural structures (think pond scum) where layers containing different groups of microbes band together. In these collections, C. aurantiacus often plays the role of anchor, helping to hold together an assortment of microbes. The bacteria, which resemble strands of string under the microscope, are usually found in hot springs, since they enjoy temperatures above 100 degrees Fahrenheit.
Wright's team performed a series of synthetic chemical steps to alter 3 vitamins that C. aurantiacus needs to survive: vitamin Bone (thiamine), vitamin Btwo (riboflavin), and vitamin Bseven (biotin). While the bacteria acknowledged the substances as normal vitamins, the researchers can monitor the mimics much more easily than their natural counterparts.
Wright's team used the mimics to relay a treasure trove of information about how vitamins enter the cell and interact within the cell, by analyzing the precise location of the molecules' activity in living cells. Through a system called affinity-based protein profiling, Wright's group effectively tagged these molecules where they're active, then used techniques such as mass spectrometry to sort and measure proteins of interest.
One of the team's findings suggests multiple vitamins may share the same molecular machinery to gain entry into the cell. The team is still investigating these data. These findings can provide a road map for scientists like Wright who are trying to direct microbes as part of broad efforts to create clean, renewable fuels and reduce the effects of climate change.
The work was funded by the D.O.E. Office of Science. The mass spectrometry-based measurements and microscopy were performed at EMSL, the Environmental Molecular Sciences Laboratory, a D.O.E. Office of Science User Facility at PNNL.
Reference: Lindsey N. Anderson, Phillip K. Koech, Andrew E. Plymale, Elizabeth V. Landorf, Allan Konopka, Frank R. Collart, Mary S. Lipton, Margaret F. Romine and Aaron T. Wright, Live cell discovery of microbial vitamin transport and enzyme-cofactor interactions, ACS Chemical Biology, Dec. 15, 2015, DOI: 10.1021/acschembio.5b00918.
Tags: Energy, Fundamental Science, EMSL, Biofuel, Chemistry, Biology, Health Science, Molecular Biology and Biochemistry, Mass Spectrometry and Separations, Microbiology
EMSL, the Environmental Molecular Sciences Lab, is a national scientific user facility sponsored by the D.O.E.'s Office of Science. Located at Pacific Northwest National Lab in Richland, Wash., EMSL offers an open, collaborative environment for scientific discovery to researchers around the world. Its integrated computational and experimental resources enable researchers to realize important scientific insights and create new technologies. Follow EMSL on Facebook, LinkedIn and Twitter.
Interdisciplinary teams at Pacific Northwest National Lab address many of America's most pressing issues in energy, the environment and national security through advances in basic and applied science. Founded in 1965, PNNL employs 4,400 staff and has an yearly budget of nearly $1 billion. It is managed by Battelle for the D.O.E.'s Office of Science. As the single biggest supporter of basic research in the physical sciences in the United States, the Office of Science is working to address some of the most pressing challenges of our time. For more information on PNNL, visit the PNNL News Center, or follow PNNL on Facebook, Google+, LinkedIn and Twitter.
